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granulosa cell line kgn  (BioResource International Inc)

 
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    BioResource International Inc granulosa cell line kgn
    Granulosa Cell Line Kgn, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granulosa cell line kgn/product/BioResource International Inc
    Average 90 stars, based on 1 article reviews
    granulosa cell line kgn - by Bioz Stars, 2026-03
    90/100 stars

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    Cytotoxic effects of graphene oxide nanosheets on <t>KGN</t> cells. ( A ) Human <t>ovarian</t> <t>granulosa</t> cells were cultured and exposed to graphene oxide nanosheets (0.1–100 μg/mL) at varying concentrations for 48 h and 72 h, respectively. Subsequently, a CCK-8 assay was conducted to determine the percentage of viable cells that were expressed. ( B ) Flow cytometry was used to analyze apoptosis in KGN cells treated with Annexin V-FITC/PI after 72 h of different treatments. ( C ) Quantitative analysis of the ratio of apoptosis and cell necrosis in ( B ). Data are expressed as Mean ± SEM (n=3). Statistical differences between experimental groups were analyzed by the Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
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    Cytotoxic effects of graphene oxide nanosheets on <t>KGN</t> cells. ( A ) Human <t>ovarian</t> <t>granulosa</t> cells were cultured and exposed to graphene oxide nanosheets (0.1–100 μg/mL) at varying concentrations for 48 h and 72 h, respectively. Subsequently, a CCK-8 assay was conducted to determine the percentage of viable cells that were expressed. ( B ) Flow cytometry was used to analyze apoptosis in KGN cells treated with Annexin V-FITC/PI after 72 h of different treatments. ( C ) Quantitative analysis of the ratio of apoptosis and cell necrosis in ( B ). Data are expressed as Mean ± SEM (n=3). Statistical differences between experimental groups were analyzed by the Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
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    Cytotoxic effects of graphene oxide nanosheets on <t>KGN</t> cells. ( A ) Human <t>ovarian</t> <t>granulosa</t> cells were cultured and exposed to graphene oxide nanosheets (0.1–100 μg/mL) at varying concentrations for 48 h and 72 h, respectively. Subsequently, a CCK-8 assay was conducted to determine the percentage of viable cells that were expressed. ( B ) Flow cytometry was used to analyze apoptosis in KGN cells treated with Annexin V-FITC/PI after 72 h of different treatments. ( C ) Quantitative analysis of the ratio of apoptosis and cell necrosis in ( B ). Data are expressed as Mean ± SEM (n=3). Statistical differences between experimental groups were analyzed by the Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.
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    Cytotoxic effects of graphene oxide nanosheets on KGN cells. ( A ) Human ovarian granulosa cells were cultured and exposed to graphene oxide nanosheets (0.1–100 μg/mL) at varying concentrations for 48 h and 72 h, respectively. Subsequently, a CCK-8 assay was conducted to determine the percentage of viable cells that were expressed. ( B ) Flow cytometry was used to analyze apoptosis in KGN cells treated with Annexin V-FITC/PI after 72 h of different treatments. ( C ) Quantitative analysis of the ratio of apoptosis and cell necrosis in ( B ). Data are expressed as Mean ± SEM (n=3). Statistical differences between experimental groups were analyzed by the Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Graphene Oxide Nanosheets Induce Mitochondrial Toxicity in Human Ovarian Granulosa Cells: Implications for Female Reproductive Health

    doi: 10.2147/IJN.S495259

    Figure Lengend Snippet: Cytotoxic effects of graphene oxide nanosheets on KGN cells. ( A ) Human ovarian granulosa cells were cultured and exposed to graphene oxide nanosheets (0.1–100 μg/mL) at varying concentrations for 48 h and 72 h, respectively. Subsequently, a CCK-8 assay was conducted to determine the percentage of viable cells that were expressed. ( B ) Flow cytometry was used to analyze apoptosis in KGN cells treated with Annexin V-FITC/PI after 72 h of different treatments. ( C ) Quantitative analysis of the ratio of apoptosis and cell necrosis in ( B ). Data are expressed as Mean ± SEM (n=3). Statistical differences between experimental groups were analyzed by the Student’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

    Article Snippet: The ovarian granulosa cell line KGN has been widely used as an in vitro model for various studies related to female reproductive function., The human ovarian granulosa cell line KGN was a gift from Associate Professor Yajing Liu of Anhui Medical University.

    Techniques: Cell Culture, CCK-8 Assay, Flow Cytometry